40ft. High throughput sequencing techniques work by generating large libraries of short (50-200 bases, usually) DNA fragments and determining their sequences in parallel. Why are flanking sequences important for amplifying STR fragments? This is actually an important point. Transgene cassettes that are inserted into bacterial plasmids are called chloroplast vectors and they are bombarded into plant cells using gold particles and a gene gun (Fig. The density of RAD tags in a genome depends on the restriction enzyme used during the isolation process. Why are flanking sequences important for amplifying STR fragments? 3b). 10. These primers are then used to amplify the STR fragment from 10 different elephants. Would you expect the fragment to be the same size in all the elephants? ... Flanking regions are important because knowing their sequences enables geneticists to isolate the STR using polymerase chain … 11. 11. This is really a fascinating question of tactics and the correct answer depends on what armies in what time period under what leadership are facing one another! A genomic clone of about 3.0 kb was isolated and sequenced. Highly divergent N‐ and C‐terminal flanking sequences are important for the function of sHsps, as the isolated α‐crystallin domains investigated so far show no chaperone activity (22 – 24). The 5′ flanking region of the human GR was first isolated and characterized by Zong et al. of movement is enough to run almost a complete circle around a medium creature, a size of creature most players are expected to face most of the time. Answer: very STR has multiple alleles, or variants, each defined by the number of repeat units present or by the length of the sequence. We confirm the important role of flanking sequences using binding assays. Chloroplast genome sequences are essential to build transgene cassettes because they provide both flanking and regulatory sequences. Would you expect the fragment to be the same size in all the elephants? Starting from a limited amount of known sequence to identifying the sequences flanking it, is a challenge relevant to many analyses (Table 1). 30ft. These primers are then used to amplify the STR fragment from 10 different elephants. Of course you didn’t really expect a simple answer did you? That is why you need a good army composition. Notice that with each stack, my infantry outnumber both my Cavalry and My artillery. 10. A scientist makes primers specific to a particular STR fragment in elephants. Isolation of RAD tags. Explain your answer. for a forty stack, I do 20 Infantry, 8 Cavalry, and 12 Artillery. The structures show that the amino acids N-terminally flanking the basic cluster make specific contacts with the receptor that are distinct from the interactions between bipartite NLSs and Impα. 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