Branden, Carl I., and John Tooze.
McGraw-Hill Medical, 2011. Jump to Today. This paper deals with the engineering drawing preparations and interpretations. Python in a Nutshell. Rs. The assessments for the course are divided into external examination, internal assessment, labs and workshops and industrial training which are taken twice by the students after fourth and sixth semester. The focus is to provide an understanding of the functions of various biomolecules and their metabolism.
We develop novel techniques that combine ideas from mathematics, computer science, probability, statistics, and … Overall hours.
Computational Biology is a multidisciplinary approach to applying data-scientific methods, processes, or theories to the study of biological systems.
INR 8 LPA
[Preview with Google Books]. Compared to DIT Dehradun, NIT Sikkim is a more preferable option.Read more, • Top Answer By Pratyasha on 10-Dec-2020, Ans.
Alberts, Bruce, Alexander Johnson, et al.
Overview of Immune System, Antibody Structure and Functions, B Cell Function, Antigen- Antibody Interactions, Cell Maturation, Activation and Differentiation, Immune System in Health and Disease. This paper deals with the analytical abilities in mathematical solutions.
Computational Biology or Master of Science in Computational Biology is a postgraduate Biology.Course includes Introduction to Life Sciences, Bio Mathematics & Statistics, Bio-Physics, Computational Genomics, Introduction to Biotechnology, Computational Proteomics & Metabolemics, Computational Transcriptomics, Computational Molecular Phylogenetics, Advanced Programming … The cell biology deals with the workings of the cells in a body and related aspects to it. CSE at PES University will be a good career choice and worth the investment. ), Learn more at Get Started with MIT OpenCourseWare, MIT OpenCourseWare makes the materials used in the teaching of almost all of MIT's subjects available on the Web, free of charge. But wait, there’s more! Studying the virus in real time.
ChemGenome: Chemgenome identifies protein coding / regulatory regions from genomic information based on the physico-chemical principles behind genome organization. MPhil Bioinformatics & CADD at the Department of Computational Biology & Bioinformatics, University of Kerala is a innovative programme, being the first of its kind in India. Civil Engineering: While the course is well-designed, the placements for this branch are not up to the mark.
Introduction, Perceptrons, Pattern Associators and Feedforwarding Networks, Extension and Advanced Topics, Competitive and Self- Organisation Networks.
A very useful introduction or refresher. The deep understanding of the concepts related to artificial intelligence. Courses Making students aware of the different database management systems and how to manage them. Computational Biology # CBIO-616 and CBIO-627 are prerequisite for $ CBIO … Structure of Nucleic Acid, DNA Replication and Mutation, Gene Expression and Regulation, Mendelian Genetics, Crossing over and Linkage. ISBN: 9781429235020. Authentic student reviews helped me compare colleges easily. Therefore, students who do not have an issue with location and are looking for a premium heritage university can opt for NIT Sikkim. Syllabus Masters Programme in Bioinformatics (Two Years) MMV, BHU Semester 1 Fundamentals of Programming Languages Essential Mathematics and Statistics I Fundamentals of Bioinformatics Biochemistry, Cell Biology … BCB 850.
ISBN: 9780071781503. ISBN: 9780521629713.
Through biological analysis and high-performance computing, critical contributions are made to basic biology…
Chapman and Hall / CRC, 2013. Our students build a solid foundation through course works in mathematical tools in biology, signal and image analysis, Bioinformatics, Computational Biology, Genomics, Proteomics, Machine Learning, and Clinical Informatics.
With three NIH Training Grants, we offer three degree programs (Ph.D., Master’s and Accelerated Master’s) fit various career goals of our students. The M.S. Graduate versions of the course involve an additional project component. The application of information technology in the medical field.
Elementary Biophysics, DNA Biophysics, Protein Biophysics, Carbohydrate Biophysics, Techniques in Biophysics. The EE branch at NIT Agartala is known to be one of the best branches the institute has to offer. This course is an introduction to computational biology emphasizing the fundamentals of nucleic acid and protein sequence and structural analysis; it also includes an introduction to the analysis of complex biological systems.
With more than 2,400 courses available, OCW is delivering on the promise of open sharing of knowledge. The 3 colleges receive varying degrees of exposure. Max ECTS 48. This paper focuses on providing an elementary knowledge in bioinformatics and biological information. The course fee is 13.2 lakh.Read more, • Top Answer By Supriya Sarkar on 10-Dec-2020, Ans. Students in one of the graduate versions of this course will complete a computational biology research project.
Five written or computer-based problem sets will be assigned. All students will provide online feedback (peer review) on the presentations. This is one of over 2,200 courses on OCW. Introduction to Protein Structure. The purpose is to make the students understand the general principles of modern approaches to treat genetic diseases and cancer. CSE is a better option for the students due to its outstanding market acceptability & demand. Introduction, Control Statements, Classes, Methods and Inheritance, Packages, Applets and String Handling, Event Handlings and AWT Controls, Applications of JAVA. Curve Fitting and Numerical Solution of Equations, Finite Differences and Interpolation, Numerical Differentiation and Integration, Numerical Solutions of Ordinary Differential Equations, Numerical Solutions of Partial Differential Equations. The course deals with ideas about circuit analysis, working principles of machines and measuring instruments. October 2, 2020. The paper is a study of the artificial neural networks and its applications.
It is ranked 93 for BTech by NIRF 2020. The Computational Biology Graduate Group facilitates student immersion into UC Berkeley’s vibrant computational biology research community.
Multiple Integrals and Differential Equations. The basics of computational neuroscience are the main focus of this paper. The paper deals with the fundamentals of chemo-informatics and applications of computer aided designing of drugs. The facility is providing free access of its Bioinformatics and Computational Biology tools to the global user community and free Supercomputing time to Students & Reseachers with-in the country. UNC Bioinformatics and Computational Biology Student Michael Lafferty Selected for Human Genomics Program. Electronic Components, Semiconductor Devices, Transducers, Digital Electronics, Communication Systems. History, Scope and Importance, Databases- Tools and their Uses, Sequence Alignment Methods, Predictive Methods Using DNA and Protein Sequences, Drug Discovery Process. In terms of placements, tabulated below is a brief idea on both the institutions:
Introduction, Greedy Algorithms, Dynamic Programming Algorithms, Graph Algorithms, Clustering and Trees. NIT Sikkim is also great for those who are looking for a degree that holds national importance. Other details are mentioned in the table below:
Fundamentals of Spectroscopy, X-Ray Chrystallography, Circular Dichroism, Optical Rotary Dispersion and Fluorescence Polarization, Principles of Nuclear Magnetic Resonance and Electron Spin Resonance, Mass Spectrometry. Find materials for this course in the pages linked along the left. Ethical Issues, Research Methodology and Intellectual Property Rights. Collegedunia is a one-stop solution to all your education related queries. Introduction to C-Programming, OOP Concepts and C++, C++, Application in Bioinformatics. PES-IUP (Indiana University of Pennsylvania) which will have 6 months to be studied in the US. Electronics Engineering: The faculty is really good and more than 90% of students are placed with the highest CTC of 12 LPA. Water Treatment, Polymers and Reinforced Plastics, Phase Equilibrium, Lubricants and Adhesives, Corrosion and its Control, Instrumental Methods of Analysis.
Training in Bioinformatics and Computational Biology Teaching. INR 18.5 LPA
Welcome to the official website of the Department of Computational Biology and Bioinformatics, University of Kerala. NIT Sikkim
Achievements is Biotechnology and Bioinformatics, Introduction to new horizons in Biotechnology and Bioinformatics, Plant and Agriculture Biotechnology, Industrial, Food and Medical Biotechnology, Environmental Biotechnology, Bioaugmentation, Biostimulation, Current State of Genetically Modified Organism. 48 LPA
Vectors and Insert, Cloning Technique, Construction of Gene Libraries, Expression of Recombinant Protein in E. Electronic and Photonic Materials, Magnetic and Dielectric Materials, Modern Engineering and Biomaterials, Introduction to Nanoscience and Nanotechnology, Materials Characterisation. Nearly 90% of students are placed with the highest CTC of 17 LPA. Leach, Andrew. INR 13 LPA
The students are also required to take a compulsory internship after the completion of the sixth semester as a part of the curricula and they shall be evaluated accordingly. So, for a management course PES University will be a better option. Module aims. Prerequisite: BINF 6101, 6201 or equivalents. Prentice Hall, 2001. Bioinformatics / ˌ b aɪ. Article by Diane Stopyra Photo by iStock December 08, 2020.
See the table for clarification. Highest CTC
Matrices, Trigonometry, Differential Calculus, Integral Calculus, Applications. You will … Comp Genomics & Bioinformatics. Bioinformatics professionals develop algorithms, programs, code, and analytic models to record and store data related to biology. O'Reilly Media, Inc., 2003. of Computational Biology and Bioinformatics… oʊ ˌ ɪ n f ər ˈ m æ t ɪ k s / is an interdisciplinary field that develops methods and software tools for understanding biological data, in particular when the data sets are large and complex. This paper focuses on providing an elementary knowledge in bioinformatics and biological information. Bioinformatics and Computational Biology. Because of this factor, relatively fewer companies visit the campus for recruitments.
An introduction to topics related to biological networks from a physicist's perspective. Linear Data Structures, Non-Linear Data Structures, Sorting Methods, Graph Algorithms, Searching. Bioinformatics and computational biology courses @ UF; Video Tutorials; Events. »
ING-INF/05. Introduction, Computer Design of Oligonucleotide Probes, Normalisation, Analysis, Experimental Design. No need to remember deadlines as I get timely updates now. Introduction, Metabolism in Systems Perspective, Biological Processes, Oscillation and Evolution, Applications and Tools. Fundamentals, Projection of Lines and Solids, Sections and Developments, Pictorial Projections, Building Drawing.
The field includes foundations in Mathematics, Statistics, … Bioinformatics and Computational Biology Bioinformatics and Computational Biology. Category: Advanced Bioinformatics and Computational Biology. This course is part of a series of courses designed to cross-train life science graduate students in computational … Though the two fields are interrelated, bioinformatics and computational biology … Applications are invited for MSc and MPhil programmes at Dept.
Introduction, Spiking Neurons and Response Variability, Feed-Forward Mapping Networks, Auto- Associative Memory and Network Dynamics, Supervised Learning and Reward Systems, Case Study.
Syllabus . There's no signup, and no start or end dates. This textbook is recommended for the course: Zvelebil, Marketa J., and Jeremy O. Baum. The placement statistics for both institutions are mentioned below:
A.Y. View Syllabus.pdf from CS 612 at University of Massachusetts, Boston. CSci 5461: Functional Genomics, Systems Biology and Bioinformatics Fall 2017 Syllabus Prof. Rui Kuang (email@example.com) Course Description: The new biotechnologies developed in the past decade are transforming molecular biology research into a quantitative science based on informatics. During this course, a student has to attend various laboratory experiments which provide a practical application of the concepts taught in the classrooms. Scientists in bioinformatics and computational biology collect, store, analyze, interpret, and present complex biological data. Important Spring 2020 UNC Bioinformatics and Computational Biology Class Updates COVID-19 Updates for March 18, 2020 UNC Bioinformatics and Computational Biology Student Shengjie Chai Co-First Author in Cancer Immunology Research BMS and RNSIT are engineering colleges primarily offering good engineering courses. Highest Salary
The course will provide an introduction to genomics, bioinformatics tools, and data analysis.
“Bioinformatics is used to abstract knowledge and principles from large-scale data, to present a complete representation of the cell and the … Average Salary
Electronics and Communication: The placements within this branch are excellent, considering top companies like L&T, Infosys, Intel, Tech Mahindra are all part of the recruitment drive.
Credits: 0 Category: Core Syllabus. Internships are provided in the 5th semester and the companies like Morgan Stanley, Goldman Sachs, Itron, Cisco visit the campus. Average CTC
Siddharth Kanan, MBA Finance, RNSIT College of MBA (2019)
I was anxious about my exam preparation. Practice Paper section helped me in preparing without coaching. Molecular Biology of the Cell. Syllabus.
of Computational Biology and Bioinformatics, University of Kerala . Chemical Bonding, Theory of Chemical Bonding, Chemistry of Carbohydrate, Proteins, Stereo Chemistry, Confirmational Analysis.
The material covered on each exam is non-cumulative; consult the Calendar to see which topics were covered on each exam.
Academically, NIT Sikkim does not have any relevant offerings as compared to NIT Agartala. The course deals with the different concepts of proteomics and their analysis. Biosafety and GMOs in India, Biosafety- Regulatory Framework for GMOs, Bioethics, Research Methodology, Intellectual Property Rights.
2019 placements were huge, as 10 students from CSE backed a package of Rs. Category: Advanced Bioinformatics. Bioinformatics … Schedule.
ISBN: 9780596100469. This paper provides the analytical abilities to the students to solve the mathematical problems effectively. The introduction of immunology, the types of immune systems and their mechanism. The paper is about the in-depth knowledge of the spectroscopy methods used.
INR 7 LPA
Related News. The computational models are developed based on a thorough understanding of molecular modeling methods, data analysis techniques, machine learning, systems biology …
Biology NIT Sikkim
This course focuses on the basic concepts of mechanical engineering. The purpose of the course is to understanding the genetic algorithms and its application.
The aim of this course is to introduce the concept of python and its applications. Taylor & Francis, Inc., 2000. Syllabus (PDF) (link is external) This course provides an introduction to mathematical and computational modeling for both experimentally and theoretically inclined students, as well the currently employed strategies to investigate physiological problems with computational modeling. B.S. Massachusetts Institute of Technology. Dana-Farber Cancer Institute. Research Groups/Events.
Undergraduate preparation reflecting a balance of training in computational biology’s core disciplines (biology, computer science, statistics/mathematics), for example, a single interdisciplinary major, such as computational biology or bioinformatics; a major in a core discipline and a combination of interdisciplinary course work and research experiences; or a double major in core disciplines. 1.08 Lakhs
Mechanical Engineering: Popular for its labs and experienced faculty members, this branch observes a comparatively low placement rate since very few companies visit for the mechanical field. This paper focuses on the concepts of physics and helping students become acquainted with them.
2006. Your decision of choosing among these 3 colleges will show that PES University is a better institution for MBA. Collegedunia helped me to ace it. Introduction, Control Statements, File Parsing, Sequences and Biopython, Database search using Biopython, Advanced molecules in Biopython. It is known to be extremely reputed and offers the best academic program in the field. These are designed to promote deeper understanding of the principles and algorithms discussed in class and to provide hands-on experience with bioinformatics tools. Biological Sequence Analysis: Probabilistic Models of Proteins and Nucleic Acids. The different versions of this course have different grading schemes. We are interested in the mechanisms of gene regulation and the prediction of RNA and protein structure.
The introduction of chemical compounds that are important for chemoinformatics. In order to keep pace with one of the fastest growing fields in the country, the B.S. “This course focuses on current problems in computational biology and bioinformatics.
BMS College of Engineering
History, Scope and Importance, Databases- Tools and their Uses, Sequence Alignment Methods, Predictive Methods Using DNA and Protein Sequences, Drug Discovery Process. 70%
ISBN: 9780321762436. RNSIT
Bioinformatics Graduate Program.
Garland Science, 2007. Bioinformatics Algorithms Highest CTC
Computational Biology (Bioinformatics) STAT/BIOC 442/842, Fall 2016 Instructor: Steve (Istvan) Ladunga, Ph.D.
Topics covered in the course include principles and methods used for sequence alignment, motif finding, structural modeling, structure prediction and network modeling, as well as currently emerging research areas… Bioinformatics MSc. Single-Cell Analysis Working Group; Blogs.
The course deals with the concepts of electronic devices, their working, operation and characteristics. There are some important points to consider before choosing a college:
Currently, the Group includes over 46 … Placement Rate
Students are responsible for assistance in … Bioinformatics is an interesting field of research combining biological sciences and computer sciences. Placements start in the 7th or 8th semester of the course. Computational Biology *30 Hrs for 2 Credit paper (24 …
This integrative approach is called computational biology. “Bioinformatics is used to abstract knowledge and principles from large-scale data, to present a complete representation of the cell and the organism, and to predict computationally systems of higher complexity, such as the interaction networks in cellular processes and the phenotypes of whole organisms” (Bayat, 2002). CSE: Over 80% of students are placed but the average median salary is quite low, around INR 6 LPA. These language courses are usually French, German, and Chinese etc.
They offer MBA-VTU in 3 Specialization (i.e., Finance, Marketing, HR) and even for Dual Specialization any of the above two. Although, in terms of the other branches the placement facilities are not quite up to the mark. Introduction, Pharmacogenetics, Drug Response, Single Nucleotide Polymorphism, Sage. An additional 1% extra credit may be awarded for exceptional class participation. Understanding Bioinformatics.
Tree of life diagram. ISBN: 9781439837177. The scripting language Python—which is widely used for bioinformatics and computational biology—will be used in the problem sets. Location of Class: FXB G10 Instructor Information. The course is tailored to undergraduate students in biology. Professor of Computational Biology Department of Statistics also affiliated with the Department of Biochemistry and the School of Biological Sciences Introduction, Metabolism of Carbohydrates, Protein Metabolism, Fatty Acid Metabolism and Nucleic Acid Metabolism, Oxidative Phosphorylation. Overall Placement Rate
Office: 222 Building 14. 4 PONDICHERRY UNIVERSITY SCHOOL OF LIFE SCIENCES Centre for Bioinformatics List of Soft-Core Courses for M. Tech. No enrollment or registration. The purpose of the course is to acquaint the students with the concept of systems biology with its applications. Computational Biology # CBIO-616 and CBIO-627 are prerequisite for $ CBIO-711.
3 Credits. Computational Biology. An in-depth treatment of molecular mechanics and other modeling methods. General Principles of Gene Therapy, DNA- Based Gene Therapy, Retroviral and Adenoviral Vectors, Adeno-Associated Virus- Based Vectors, Cancer Gene Therapy.
Principles of bioinformatic and computational biology pedagogy. But it is still a better-preferred course because of its faculty and lab services and a wide range of subjects as part of the academic structure. It offers the best placements and recorded the highest CTC of 24 LPA. The course is designed such that students are made aware of the aspects of research methodology and helpful for research in bioinformatics. Computational Biology. Most students are simply offered internships along with the NIT degree. Anuj Mehta, studied B.Tech at NIT Sikkim, 2018
Students have the option to specialize in one of the three program sub-areas; Biological Sciences, Computer Science and Engineering, or Biomedical Informatics. These advances have enabled scientists to break new ground in the realms of genome assembly, analysis, alignment, computational evolutionary biology, protein … Applications are invited for MSc and MPhil programmes at Dept. Rs. Bioinformatics 101 – 2019: Introduction to methods and tools for NGS analysis Offered by the ICBR Bioinformatics core, in collaboration with the UFHCC BQS SR and HSC Library.
Topics covered in the course include principles and methods used for sequence alignment, motif finding, structural modeling, structure prediction and network modeling, as well as currently emerging research areas. *Students with Mathematical and Physical Science background are expected to choose CBIO-617 as compulsory papers. Fundamentals of Cancer Biology, Principles of Carcinogenesis, Principles of Molecular Cell Biology of Cancer, Principles of Cancer Metastasis, New Molecules for Cancer Therapy.
The focus of the paper is the different strategies of gene cloning and the application of recombinant DNA technology. … Get step-by-step explanations, verified by experts. in Bioinformatics and Computational Biology (BINF) consists of a roughly equal amount of coursework in life sciences, computer science, and mathematics. To conclude, it is preferable to take admissions only if you are a dropper. The course grade includes the student's …
... An Introduction to Bioinformatics … Because of collegedunia, all my questions regarding JEE Mains were answered. This integrative approach is called computational biology. [Preview with Google Books], Martelli, Alex. We cover both foundational topics in computational biology, and current research frontiers. »
Obtaining good placements depends on the overall performance of the student, even if the college has a 100% placement record. Rs.
The main recruiters are Denso and Intel. Placement & Internship Details
Course Description. Environmental Education and ecosystems, Environmental Pollution, Waste Management, Biodiversity and its conservation, Environmental Protection.
[Preview with Google Books].
Genomes and their Organisation, Gene Expression Profiling, Gene Regulatory Network and Microarray, Functional and Comparative Genomics, Transcriptomics.
See the table for clarification.
Sequence Analysis, Methods, MHC-I Prediction, MHC-II Prediction, B Cell Epitope Prediction and Web Sources. The Cartoon Guide to Statistics. Introduction, Database Design and the E-R Model, Relational Model and its Design Technique, Structured Query Language, Biological Database Management.
In depth CAT exam analysis available for free.
Application to MSc and MPhil Programmes. JHSPH OpenCourseWare >> Bioinformatics and Computational Biology Solutions Using R and Bioconductor This is the syllabus used in conjunction with educational content offered by JHSPH. Knowledge is your reward. Tips & Tricks.
Molecular Biology of the Gene. M.Sc. To enable successful applications of computational biology and bioinformatics approaches, Biology courses complement the quantitative foundations with a wide range of areas of modern biology such as genetics, genomics, stem cell biology, or cancer biology. The research group Computational Biology develops computational models that help to obtain qualitative and quantitative knowledge of diseases, biomedical processes and structures.
There are two in-class exams that will assess knowledge about the biology topics and computational thinking. The module is designed to develop student research skills in the broad area of computational biology. More information is in the Assignments section. English. PES University is a premiere university of Karnataka offering B.Tech in CSE.
Bioinformatics is a blend of multiple areas of study including biology, data science, mathematics and computer science. This allows the construction of complex and large biological systems. Petsko, Gregory A., and Dagmar Ringe. There is no final exam. Based on different branches, let us look at the placements specifically:
The positions learners can look forward to include Computer and Information Research Scientist, Computational Biology Professor, Software and Instrumentation Test Engineer, Bioinformatics … Bachelor of Technology [B.Tech] (Bioinformatics), For more info you can simply log on to collegedunia.com, Keep up to date with our progress by following us, https://collegedunia.com/qna/question/4434-which-is-better-ece-in-nit-sikkim-or-ee-in-nit-agartala-according-to-placements, https://collegedunia.com/qna/question/4433-which-is-better-academically-dit-dehradun-or-nit-sikkim, https://collegedunia.com/qna/question/4432-how-is-the-placement-at-nit-sikkim, https://collegedunia.com/qna/question/4431-from-which-semester-do-placements-start-for-cse-students-in-pes-university, https://collegedunia.com/qna/question/4430-which-among-these-mba-colleges-are-worth-pes-university-bms-college-or-rnsit, By submitting this form, you accept and agree to our, Bachelor of Engineering [BE] (Chemical Engineering), Bachelor of Engineering [BE] (Civil Engineering), Bachelor of Technology [B.Tech] + Master of Technology [M.Tech] (Intelligent Systems), Bachelor of Technology [B.Tech] + Master of Technology [M.Tech] (Metallurgical & Materials Science), MBA/PGDM - Masters (Business Administration), BBA/BBM - Bachelors (Business Administration), Bachelor of Animation - Bachelor (Animation), Bachelors (Animation & Graphic Design) - Bachelor (Arts), Bachelor of Physiotherapy(BPT) - Bachelor (Medical), B.Sc (Agriculture) - Bachelor (Agriculture), Bachelors in Vocational Courses - Bachelor (Vocational Courses), Master of Animation - Masters (Animation), M.Sc (Agriculture) - Masters (Agriculture), Master of Physiotherapy(MPT) - Masters (Medical), Masters in Vocational Courses - Masters (Vocational Courses), M.Phil/Ph.D in Paramedical - Doctorate (Paramedical), M.Phil/Ph.D in Medicine - Doctorate (Medical), M.Phil/Ph.D in Pharmacy - Doctorate (Pharmacy), M.Phil/Ph.D in Science - Doctorate (Science), Ph.D in Veterinary Science - Doctorate (Veterinary Sciences), M.Phil/Ph.D in Mass Communication - Doctorate (Mass Communications), M.Phil/Ph.D in Management - Doctorate (Management), M.Phil/Ph.D in Architecture - Doctorate (Architecture), M.Phil/Ph.D in Commerce - Doctorate (Commerce), M.Phil/Ph.D in Computer Applications - Doctorate (Computer Applications), M.Phil/Ph.D in Dental - Doctorate (Dental), M.Phil/Ph.D in Agriculture - Doctorate (Agriculture), M.Phil/Ph.D in Design - Doctorate (Design), M.Phil/Ph.D in Hotel Management - Doctorate (Hotel Management), M.Phil/Ph.D in Engineering - Doctorate (Engineering), M.Phil/Ph.D in Education - Doctorate (Education).
Bioinformatics for Computational Genomics (Classe LM-8)-Enrolled from 2019/2020 academic year .
The aim of the paper is to understand the structure of biomolecules. 80%
The project is designed to give you practice in applying computational methods to contemporary problems in biology.
Primer of Biostatistics. Primary and Secondary Structure, Receptors, Engineering of Macromolecules, Data Analysis Methods, Methods of Protein Engineering. 2nd ed.